Device

Part:BBa_K392045:Design

Designed by: Tadashi Nakamura, Shuhei Yasumoto, Saya Kakuda, Takahiro Saka   Group: iGEM10_Osaka   (2010-11-07)


endoglucanase secretion testing construct for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1604
    Illegal NotI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2661
    Illegal BamHI site found at 1797
    Illegal XhoI site found at 2159
    Illegal XhoI site found at 2408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 1939
    Illegal NgoMIV site found at 2864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830
    Illegal BsaI site found at 1843


Design Notes

Assembled from Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.


Source

Silver assembly of J63005, K416003, K392006, K392003.

References